Probing cell internalisation mechanics with polymer capsules.

نویسندگان

  • Xi Chen
  • Jiwei Cui
  • Yuan Ping
  • Tomoya Suma
  • Francesca Cavalieri
  • Quinn A Besford
  • George Chen
  • Julia A Braunger
  • Frank Caruso
چکیده

We report polymer capsule-based probes for quantifying the pressure exerted by cells during capsule internalisation (Pin). Poly(methacrylic acid) (PMA) capsules with tuneable mechanical properties were fabricated through layer-by-layer assembly. The Pin was quantified by correlating the cell-induced deformation with the ex situ osmotically induced deformation of the polymer capsules. Ultimately, we found that human monocyte-derived macrophage THP-1 cells exerted up to approximately 360 kPa on the capsules during internalisation.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Title of Document: POLYMER CAPSULES AS BUILDING BLOCKS FOR SOFT, CONNECTED MESOSTRUCTURES

Title of Document: POLYMER CAPSULES AS BUILDING BLOCKS FOR SOFT, CONNECTED MESOSTRUCTURES Elijah O. George, Master of Science, 2009 Directed by: Prof. Srinivasa R. Raghavan Department of Chemical & Biomolecular Engineering We show that polymer capsules can serve as soft building blocks for creating a range of mesoscale (0.1 to 10 mm) structures. The central innovation is a new approach for conn...

متن کامل

Endocytic capsule sensors for probing cellular internalization.

A new class of polymer capsules with an in-built endocytic pH-coupled fluorescence switch is reported. These capsules display reversible "on/off" fluorescence in response to cellular pH variations. Using this system, the high-throughput quantification between surface-bound and internalized capsules is demonstrated. This system allows a fundamental study of the interaction between nanoengineered...

متن کامل

Transparent Self-Healing Polymers Based on Encapsulated Plasticizers in a Thermoplastic Matrix

A self-healing approach for optically transparent thermoplastic polymers, based on plasticizer-induced solvent welding, is reported. For the specifi c system investigated, dibutylphthalate (DBP) fi lled urea-formaldehyde capsules are dispersed in a polymethylmethacrylate (PMMA) matrix. Upon a damage event, DBP is released into the crack, and locally plasticizes and swells the polymer, enabling ...

متن کامل

Coordination polymer particles as potential drug delivery systems.

Micro- and nanoscale coordination polymer particles can be used for encapsulating and delivering drugs. In vitro cancer cell cytotoxicity assays showed that these capsules readily release doxorubicin, which shows anticancer efficacy. The results from this work open up new avenues for metal-organic capsules to be used as potential drug delivery systems.

متن کامل

The role of capsule stiffness on cellular processing† †Electronic supplementary information (ESI) is available. See DOI: 10.1039/c5sc00416k Click here for additional data file.

Particle stiffness is emerging as an important parameter in determining the cell uptake dynamics of particles. Understanding the effects of capsule stiffness on their biological behavior is essential for the development of polymer capsules as therapeutic carriers. Herein, we report the preparation of polysaccharide capsules via atom transfer radical polymerization-mediated continuous assembly o...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Nanoscale

دوره 8 39  شماره 

صفحات  -

تاریخ انتشار 2016